AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.
机构:
Beijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R ChinaBeijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R China
Xu, WeiDong
Jing, HuaYong
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Beijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R ChinaBeijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R China
Jing, HuaYong
Zhang, FuLi
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Beijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R ChinaBeijing PLA, Mil Gen Hosp, Dept Radiat Oncol, 5 Nanmencang, Beijing 100700, Peoples R China
Zhang, FuLi
FRONTIERS IN BIOSCIENCE-LANDMARK,
2016,
21
: 410
-
418
机构:
Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Sartore-Bianchi, Andrea
Sporchia, Andrea
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Sporchia, Andrea
Cerea, Giulio
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Cerea, Giulio
Maugeri, Maria Rosaria
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Maugeri, Maria Rosaria
Cipani, Tiziana
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Cipani, Tiziana
Andreotti, Ines
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Andreotti, Ines
Marrapese, Giovanna
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Marrapese, Giovanna
Artale, Salvatore
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
Artale, Salvatore
Siena, Salvatore
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Osped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, ItalyOsped Niguarda Ca Granda, Div Oncol Falck, Piazza Osped Maggiore 3, I-20162 Milan, Italy
机构:
Univ Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Unit 432, Houston, TX 77030 USAUniv Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Unit 432, Houston, TX 77030 USA
Morgillo, F
Lee, HY
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Univ Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Unit 432, Houston, TX 77030 USAUniv Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Unit 432, Houston, TX 77030 USA