Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently

被引:0
|
作者
Jing-Hua Kuai [1 ]
Qing Wang [1 ]
Ai-Jun Zhang [1 ]
Jing-Yu Zhang [1 ]
Zheng-Feng Chen [1 ]
Kang-Kang Wu [1 ]
Xiao-Zhen Hu [2 ]
机构
[1] Department of gastroen- terology,Qilu Hospital of Shandong University
[2] Department of general Surgery,Qilu Hospital of Shandong University
关键词
Epidermal growth factor receptor; Immune magnetic liposomes; Epithelial cell adhesion molecule; Circulating tumor cells; Colorectal cancer;
D O I
暂无
中图分类号
R735.34 [];
学科分类号
100214 ;
摘要
AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.
引用
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页码:351 / 359
页数:9
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