IL-25在小鼠哮喘中通过Nuocyte细胞诱导Th2炎性细胞因子的表达

被引：5

作者：

刘清发 ^{[1
]}

陈方方 ^{[1
]}

刘粉 ^{[1
]}

李鸿佳 ^{[1
]}

许文娟 ^{[2
]}

孙启晶 ^{[1
]}

宫冰 ^{[3
]}

张才擎 ^{[1
]}

机构：

[1] 山东大学医学院附属千佛山医院呼吸内科

[2] 泰山医学院

[3] 潍坊医学院

来源：

基础医学与临床 | 2015年 / 35卷 / 11期

关键词：

IL-25; 哮喘; nuocyte细胞; IL-5; IL-13;

D O I：

10.16352/j.issn.1001-6325.2015.11.016

中图分类号：

R562.25 [];

学科分类号：

摘要：

目的研究哮喘气道炎性因子IL-25通过nuocyte细胞对Th2炎性细胞因子的影响。方法建立小鼠哮喘模型,分离并培养nuocyte细胞,将nuocyte细胞分为PBS对照组、IL-25处理组、抗IL-25组,体外给予IL-25和抗IL-25处理。ELISA方法检测细胞上清液IL-5和IL-13的含量;Western blot检测细胞IL-5和IL-13蛋白表达;RT-PCR检测IL-5和IL-13 mRNA表达;流式细胞计数测定nuocyte细胞数量。结果 IL-25处理组nuocyte细胞IL-5及IL-13在蛋白和基因水平的表达均比对照组和抗IL-25组IL-5及IL-13表达水平高(P<0.05);IL-25处理组nuocyte细胞数量高于对照组及抗IL-25组(P<0.05)。结论 IL-25在小鼠哮喘中可通过nuocyte细胞诱导IL-5和IL-13的表达,促进哮喘的发生和发展。

引用

页码：1508 / 1513

页数：6

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