Wnt拮抗基因SFRP1、SFRP2启动子区甲基化与贲门腺癌关系的研究

被引：1

作者：

董稚明 ^{[1
]}

王馥丽 ^{[2
]}

靳国梁 ^{[3
]}

郭炜 ^{[1
]}

郭艳丽 ^{[1
]}

王士杰 ^{[4
]}

机构：

[1] 河北省肿瘤研究所病理研究室

[2] 河北省唐山市开滦医院病理科

[3] 河北省磁县肿瘤医院病理科

[4] 河北医科大学第四医院胃镜室

来源：

肿瘤研究与临床 | 2009年 / 12期

关键词：

胃肿瘤; 基因; 甲基化; SFRP1; SFRP2;

D O I：

暂无

中图分类号：

R735 [消化系肿瘤];

学科分类号：

100214 ;

摘要：

目的研究贲门腺癌(GCA)中分泌型卷曲相关蛋白1(SFRP1)、分泌型卷曲相关蛋白2(SFRP2)基因启动子区甲基化状态与贲门腺癌的关系。方法采用甲基化特异性PCR(MSP)方法检测GCA癌组织及相应癌旁正常组织中SFRP1和SFRP2基因的甲基化状态,分析其与临床病理特征之间的关系。结果 94例GCA组织中SFRP1、SFRP2基因甲基化率分别为87.2%(82/94)和83.0%(78/94),显著高于癌旁正常组织14.9%(7/47)和55.3%(26/47)(P<0.001)。GCA患者中无淋巴结转移组SFRP1基因甲基化发生率73.7%(28/38)显著低于有淋巴结转移组的甲基化率96.4%(54/56)(P<0.05);SFRP2基因的甲基化与有无淋巴结转移无关。SFRP1和SFRP2基因的甲基化与GCA的组织分化无关。63例贲门腺癌组织中SFRP1和SFRP2基因同时发生甲基化,其中有淋巴结转移的36例高于无淋巴结转移的27例,高中分化腺癌组26例,低于低分化腺癌组37例,侵及肌层及浆膜层的16例低于侵及周围软组织的47例,但以上差异均无统计学意义(P>0.05)。结论 SFRP1、SFRP2基因可能参与了贲门腺癌的发生发展,并且SFRP1高甲基化状态与贲门腺癌的恶性行为有关。

引用

共 6 条

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