DINUCLEOSIDE MONOPHOSPHATE ACTING AS THE SHORTEST ACCEPTOR IN T4 RNA LIGASE REACTION

A thorough study on the reaction of 32Nwith 14 dinucleoside monophosphates including UI and CmI has been reported. The 10μl reaction mixture containing 100mM Tris （pH8.3）, 40mMMgCl, 6.6mM DIT, bovine serum albumin （20μg/ml）, 1mM ATP, 0.25mM dinucleoside monophos-phate, 0.05mM 32Nand TRNA ligase was incubated for 24 hr at 5--10℃ （or 2 hr at 37℃）.The joining yields depend on the reaction temperature, the donor species and the base compositionof acceptors,Cis the best donor. At 5--10℃, it can join in all 14 dinucleoside monophosphateswith yields ranging from 20% to 80%. The next one isA, but the yields are comparatively low.BothGand pare poor donors, which react with some dinucleoside monophosphates to give ayield of only a few percent, and sometimes no product could be obtained whatsoever.