Effect of Forskolin on Progesterone and Plasminogen Activator Secretion by Perfused Rat Corpora Lutea during Pseudopregnancy

Effects of forskolin on progesterone and plasminogen activator production in pseudopregnant ratcorpora lutea was investigated using isolated in vitro perfused ovaries.Progesterone andplasminogen activator production were measured on day 1,8 and 18 of hCG-inducedpseudopregnancy.The results indicated:different concentrations of forskolin(100,200,400 and800 μg)administered to ovaries on the 8th day of pseudopregnancy caused elevation of progesteronesecretion in a dose-dependent manner.After 8 hours of perfusion,PA contents increasedsignificantly in ovaries treated with forskolin.With exogenous PA-urokinase(800 U)added to theperfusion solution,progesterone secretion increased significantly as compared to control group andremained on high level throughout the perfusion period.Though exerting no apparent effects in lowdosage(5 mM),AMCHA,a PA inhibitor,administered in higher dosage(10 and 15 mM)led tomarked reduction in PA activity and progesterone secretion as compared to control group.Thusforskolin causes significant elevation of level of progesterone secretion and PA activity inpseudopregnant rat ovary perfused in vitro.And PA seems to regulate progesterone secretion in theperfused rat corpora luteum.