EXPERIMENTAL STUDIES ON A NEW TYPE CONCENTRATED ANTICOAGULANT HEMODIALYSATE OF CITRATE IN DOGS

Objective To observe the hemodialysis effect of the new type of concentrated anticoagulant hemodialysate of citrate. Methods Ten dogs were given intermittent hemodialysis and were divided into 3 groups according to hemodialysis manners. Group 1 was saline-flush hemodialysed with bicarbonate hemodialysate; Group 2 was hemodialysed with citrate hemodialysis without any anticoagulant; Group 3 was hemodialysed with bicarbonate hemodialysate and heparin .ACT, Ca 2+, BUN, Cr,ALT, AST, TBIL, DBIL, Na +, Cl -, HCO 3 - and venous pressure were monitored in the animals of each group during hemodialysis. Results During the hemodialysis in Group 1,venous pressure increased lastingly, resulting in the failure of hemodialysis for 2 hours. Hemodialysis for 2 hours in Group 2 were all finished successfully. ACT was extended and Ca 2+ decreased obviously in the venous end during hemodialysis.And ALT?AST?Ca 2+?K +?Na +?Cl -?HCO 3 - after the hemodialysis in Group 2 were not changed(P>0.05).Moreover, the clearance rate of the dialyzers with citrate dialysate increased significantly compared with those of saline-flush and heparin anticoagulation.Conclusion The anticoagulant and dialytic effects of the new type citrate hemodialysis are satisfactory and better than that of saline-flush