Regulation of Tau Protein on the Antidepressant Effects of Ketamine in the Chronic Unpredictable Mild Stress Model

Tau protein is known to play an important role in maintaining microtubule assembly and stabilization,and maintaining the normal morphology of neurons,but several studies have found that chronic stress leads to Tau hyperphosphorylation. A large number of clinical trials have found that ketamine,which is an N-methy-D-aspartate receptor antagonist,produces a rapid,long-lasting,and potent antidepressant effect in patients suffering from major depression. This rapid antidepressant effect of ketamine,which involves many mechanisms,has attracted wide attention. However,the relationship between ketamine’s antidepressant effects and Tau protein has rarely been examined. We used C57 BL/6 and Tau KO mice exposed to 42 days of chronic unpredictable mild stress(the CUMS model) to investigate the effect of ketamine on behavioral changes and synaptic functioning of the hippocampus. The results showed that a single treatment of ketamine rapidly relieved the CUMS-induced anhedonia,depression-like,and anxious behaviors of the C57 BL/6 mice. The abnormal behaviors were accompanied by increased levels of specific alterations of hyperphosphorylated Tau protein in cytoplasm and synapse in the hippocampus of the C57 BL/6 mice,but ketamine reduced the aggregation of hyperphosphorylated Tau protein only in the synapse. We also found that CUMS exposure reduced the levels of GluA1 and PSD95 in the hippocampus of the C57 BL/6 mice and that these deficits were reversed by ketamine. However,the Tau KO mice did not develop any stress-induced depressive behaviors or deficits of hippocampal function. The antidepressant effect of ketamine may decrease the levels of hyperphosphorylated Tau protein in synapse of C57 BL/6 mice.