Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B

Objective:To determine whether salvianolic acid B(Sal B) exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis.Methods:RSC96 cells were primarily cultured with DMEM(5.6 mmol/L glucose),hyperglycemia(HG,125 mmol/L glucose) and Sal B(0.1,1,and 10μ mol/L).Cells proliferation was measured by 3-(4,5-cimethylthiazol-2-yl)-2,5-dilphenyltetrazolium bromide assay.Reactive oxygen species(ROS) generation and apoptosis rate were detected by flow cytometry analysis.Western blot was performed to analyze the expressions of poly ADP-ribose polymerase(PARR),cleaved-caspase 3,cleavedcaspase 9,Bcl-2,Bax,NLRP3, ASC,and interleukin(IL)-1 β.Results:Treatment with HG at a concentration of125 mmol/L attenuated cellular proliferation,while Sal B alleviated this injury(P<0.05).In addition,Sal B inhibited HG-induced ROS production and apoptosis rate(P<0.05).Furthermore,treatment with Sal B down-regulated HG-induced PARP,cleaved-caspase 3,cleaved-caspase 9,Bax,NLRP3,ASC,and IL-1β expression,but mitigated HG-mediated down-regulation of Bcl-2 expression(P<0.05).Conclusion:Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.