[Objective] This study was to investigate the fat and protein components in seeds of wild Elaeagnus angustifolia from Xinjiang Uigur Autonomous Region. [Method] GC-MS was employed to measure the content and composition of fatty acids in the seeds of E. angustifolia, and fractional extraction method to isolate protein and semi micro-quantitative nitrogen method to determine amino acid components. [Result] Fat content in E. angustifolia seeds was 8.20% (with unsaturated fatty acids accounting for the dominant proportion), of which the fatty acid composition was in order linoleic acid (46.52%), oleic acid (33.17%), linolenic acid (9.21%) and stearic acid (5.31%). Mean content of protein in E. angustifolia seeds was 11.31%, of which globulin and albumin account for 70%, while the contents of residue protein, gliadin and glutenin were lower. Eighteen kinds of amino acids were found in the proteins of E. angustifolia seeds, of which Asp content was the highest (12.70%), followed by Glu (11.80%), Tyr (9.33%), Leu (8.11%), Lys (7.22%), Arg, Ser, Ala, Val, His, Met and Cys. In detail, the content of essential amino acids of human body reached 28.76%. [Conclusion] Seeds of E. angustifolia from Xinjiang Uigur Autonomous Region are full of nutrients, having a promising future.