Next-generation sequencing for clinical HLA typing

被引：0
|
作者：
Chengyu Wu [1 ,2 ]
Qiang Shi [3 ]
Dinh Pham [1 ]
Afzal Nikaein [1 ]
机构：
[1] Texas Medical Specialty,Inc.  2. Baylor Scott & White Medical Center
[2] Division of Transplantation,Department of Surgery,School of Medicine and Public Health,University of Wisconsin-Madison
来源：
实用器官移植电子杂志 | 2018年 / 6卷 / 04期
关键词：
HLA分型; 下一代测序; 全基因组Illumina数据分析; 临床应用;
D O I：
暂无
中图分类号：
R440 [];
学科分类号：
100208 ;
摘要：
下一代测序(NGS)已经被证明可有效的减少人类白细胞抗原(HLA)分型的不准确性和检测成本,同时还可以检测出之前未测序的HLA基因的详细信息。本研究介绍了在Illumina公司的Mi Seq平台上使用NGS开发的HLA分型测定的性能要求。共纳入288个样品,其之前以HLA-A,HLA-B,HLA-C,HLA-DRB1,HLA-DQA/B和HLA-DPA/B为特征,其使用Sanger测序、序列特异性引物和序列特异性寡核苷酸技术进行高分辨率的分型。这些样本携带高比例HLA特异性的等位基因。测序数据使用Omixon的HLA TwinTM进行分析。评估等位基因平衡、敏感性、特异性、精确性、准确性和不准确性。这些结果证明了NGS对HLA分型的可行性和获益处,因为这项技术非常准确,几乎排除了所有的不确定性,为HLA基因长度提供了完整的测序信息,并形成了利用单一方法进行HLA分型的基础免疫遗传学实验室。
引用
收藏
页码：275 / 285
页数：11
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