DNase1L3的活性检测及其临床初步观察

被引：0

|

作者：

金宇扬 ^{[1
]}

涂洋 ^{[2
]}

宋睿 ^{[3
]}

陈晓翔 ^{[3
]}

张彦东 ^{[4
]}

机构：

[1] 吉林大学临床医学院

[2] 同济大学附属同济医院风湿科

[3] 上海交通大学医学院附属仁济医院风湿科

[4] 吉林大学第一医院风湿免疫科

来源：

现代免疫学 | 2020年 / 40卷 / 01期

关键词：

DNA酶1样分子3; 系统性红斑狼疮; 抗双链DNA抗体; 抗核小体抗体; 分子克隆;

D O I：

暂无

中图分类号：

R593.241 [];

学科分类号：

摘要：

DNA酶1样分子3(deoxyribonuclease 1 like 3,DNase1L3)在SLE发病机制中起重要的保护作用,但目前尚缺乏活性良好的DNase1L3蛋白,为了获得有良好活性的DNase1L3并观察DNase1L3的临床价值,克隆DNase1L3分子,并筛选出稳定表达有良好活性DNase1L3的真核细胞株,选择80例SLE患者为SLE组,34例健康志愿者为对照组,检测对照组及SLE组血清DNase1L3的活性水平,同时分析SLE患者血清DNase1L3活性水平与临床指标的相关性。结果显示,SLE组患者血清DNase1L3的活性水平[(97.94±0.19)%]显著低于对照组[(98.91±0.09)%,P <0.05];虽然血清DNase1L3活性与抗核小体抗体和抗dsDNA抗体水平没有显著关联,但在DNase1L3活性<98%的患者群体中,DNase1L3活性与抗核小体抗体水平呈显著负相关(r=-0.493 1,P=0.012 3)。提示DNase1L3活性水平与SLE的疾病活动性相关,且在SLE发病机制中发挥潜在的保护作用。

引用

收藏

页码：22 / 27

页数：6

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