Effect of Ephrin-A1/EphA2 on Invasion of Trophoblastic Cells

The effect of axon guidance factors ephrin-A1/EphA2 on the invasion of trophoblastic cells and the possible mechanism were investigated in this study.The expression of EphA2 in vascular endo-thelial cells was detected by immunohistochemistry.The proliferation and invasion of TEV-1 cells(an extravillous trophoblastic cell line)in first trimester were determined by cell counting kit-8(CCK-8)and Transwell invasion assay.Real-time PCR was used to detect the expression of ephrin-A1 in TEV-1 cells treated with EphA2 at different concentrations(10,50,100,500,1000 and 5000 μg/L).The results showed:(1)EphA2 was expressed in the vascular endothelial cells;(2)EphA2 could promote the proli-feration of TEV-1 cells.The proliferative capacity reached a peak in TEV-1 cells treated with 100 μg/L EphA2(P<0.05);(3)EphA2 could increase the invasion of TEV-1 cells.The invasive ability was the greatest in TEV-1 cells treated with 500 μg/L EphA2(P<0.05);(4)in the presence of EphA2(0-500 μg/L),the expression of ephrin-A1 was increased concentration-dependently(P<0.05),but when the concentration of EphA2 was over 500 μg/L,the expression of ephrin-A1 ceased to increase(P>0.05).It was concluded that EphA2 can promote the invasion and proliferation of the human extravillous tro-phoblastic cells probably by regulating the ephrin-A1 ligand.