Papaya improves non-alcoholic fatty liver disease in obese rats by attenuating oxidative stress, inflammation and lipogenic gene expression

BACKGROUND Non-alcoholic fatty liver disease(NAFLD) is a global health issue that is correlated with obesity and oxidative stress.AIM To evaluate the anti-NAFLD effect of papaya in high fat diet induced obesity in rats.METHODS Four-week-old male Sprague-Dawley rats were divided into four groups after 1 wk of acclimatization: Group 1 was the rats fed a normal diet(C); group 2 was the rats fed a high fat diet(HFD); group 3 was the rats fed a HFD with 0.5 m L of papaya juice/100 g body weight(HFL), and group 4 was the rats fed a HFD with 1 m L of papaya juice/100 g body weight(HFH) for 12 wk. At the end of the treatment, blood and tissue samples were collected for biochemical analyses and histological assessment.RESULTS The results of the HFH group showed significantly reduced body weight(HFH vs HFD, P < 0.01), decreased NAFLD score(HFH vs HFD, P < 0.05), and reduced hepatic total cholesterol(HFL vs HFD, P < 0.01; HFH vs HFD, P < 0.001), hepatic triglyceride(HFH vs HFD, P < 0.05), malondialdehyde(HFL, HFH vs HFD, P < 0.001), tumour necrosis factor-α(HFH vs HFD, P < 0.05) and interleukin-6(HFH vs HFD, P < 0.05) when compared to the HFD group. However, the liver weight showed no significant difference among the groups. The activities of catalase and superoxide dismutase significantly increased in HFH when compared with the HFD group(P < 0.05 and P < 0.001, respectively). The suppression of transcriptional factors of hepatic lipogenesis, including sterol regulatory elementbinding protein 1 c and fatty acid synthase, were observed in the papaya treated group(HFH vs HFD, P < 0.05). These beneficial effects of papaya against HFDinduced NAFLD are through lowering hepatic lipid accumulation, suppressing the lipogenic pathway, improving the balance of antioxidant status, and lowering systemic inflammation.CONCLUSION These current results provide experimental-based evidence suggesting papaya is an efficacious medicinal fruit for use in the prevention or treatment of NAFLD.