A Comparative Study of 99mTc-YIGSR and 99mTc-MIBI Uptake in Tumor Cells

To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain,which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG 3. (1) Preparation of the 99mTc-YIGSR probe: with S-Acetly-NH 3-MAG 3 as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EAC′s uptake of 99mTc-YIGSR and 99mTc-MIBI was observed at 37 ℃ and 22 ℃, respectively. (1) The labeling efficiencies of 99mTc-YIGSR and 99mTc-MIBI were (62±3) % and (96±2) %, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 ℃, the EAC′S uptake of 99mTc-YIGSR and 99mTc-MIBI reached the peak of (43.16±2.4) % and (24.4±1.8) % at 60 min, respectively; and at 22 ℃, the highest uptake was (26.5±2.1) % and (9.47±1.9) % at 60 min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.