河南省部分地区硬蜱种类鉴定及无形体携带情况调查

被引：2

作者：

张艳 ^{[1
,2
]}

彭永帅 ^{[1
]}

机构：

[1] 河南牧业经济学院动物医药学院

[2] 郑州市兽医生物制品技术重点实验室

来源：

现代牧业 | 2021年 / 5卷 / 01期

关键词：

蜱; 种类鉴定; 无形体;

D O I：

暂无

中图分类号：

S852.746 [];

学科分类号：

摘要：

在河南省部分地区30个采样点采集寄生于绵羊、山羊、牛、犬、刺猬体表的蜱355只,扩增其16S rDNA和ITS-2基因序列,根据序列比对结果,结合形态学鉴定,显示共有长角血蜱83份、微小牛蜱16份、褐黄血蜱6份。利用巢式PCR方法扩增蜱体内4种无形体(嗜吞噬细胞无形体、绵羊无形体、边缘无形体和牛无形体)的16S rRNA/msp4基因,发现所有的长角血蜱均被无形体感染,其中绵羊无形体的阳性率最高(26.5%,22/83),在1份长角血蜱样本中检测到人兽共患病原嗜吞噬细胞无形体;微小牛蜱中仅检测到边缘无形体,感染率为18.7%(3/16);褐黄血蜱中未检测到上述无形体。种系发育分析显示,得到的上述4种无形体分离株的序列均与已知的相应无形体种聚集在一起。结果表明,河南部分地区动物体表寄生的长角血蜱体内无形体感染很普遍,并有传播人兽共患的人粒细胞无形体病的风险。

引用

页码：27 / 34

页数：8

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