Optimization of Propionic Acid Feeding for Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Fed-Batch Culture of Ralstonia eutropha

被引：0
|
作者：
Longan SHANG
Jin Hwan Do
范代娣
姜岷
Ho Nam CHANG
机构：
[1] Department of Chemical Engineering
[2] Korea Advanced Institute of Science and Technology
[3] Yusong-gu
[4] Xi’an
[5] Department
[6] Korea
[7] Department of Chemical and Biomolecular Engineering of Chemical and Biomolecular Englneering
[8] Xi’an 710069
[9] Northwest University
[10] KoreaDepartment of Chemical Engineering
[11] Taejeon 305-701
[12] 373-1 Kusong-dong
[13] China
[14] Department of Chemical and Biomolecular Englneering
来源：
Chinese Journal of Chemical Engineering | 2003年 / 02期
关键词：
biodegradable polyester; feeding strategy; P(3HB-co-3HV); propionic acid; Ralstonia eutropha;
D O I：
暂无
中图分类号：
TQ325 [聚合类树脂及塑料];
学科分类号：
0805 ; 080502 ;
摘要：
The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to achieve high cell density and high 3HV yield. Effects of different feeding strategies of propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due to the propionic acid accumulation in culture broth when the feeding solution with high P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling propionic acid concentration at a low level in the separate feeding of propionic acid. An optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9kg.m-3, 1.8kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33g HV per g propionic acid.
引用
收藏
页码：100 / 103
页数：4
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