Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

被引：0
|
作者：
Li-Xin Zhu Jing Liu You-Hua Xie Yu-Ying Kong Ye Ye Chun-Lin Wang Guang-Di Li Yuan Wang State Key Laboratory of Molecular Biology
机构：
来源：
World Journal of Gastroenterology | 2004年 / 20期
基金：
国家高技术研究发展计划(863计划);
关键词：
cell; Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells; CHO;
D O I：
暂无
中图分类号：
R373.21 [];
学科分类号：
100103 ; 100705 ;
摘要：
AIM:To explore the role of hepatitis C virus(HCV)envelopeprotein 2(E2)in the induction of apoptosis.METHODS:A carboxyterminal truncated E2(E2-661)wastransiently expressed in several cultured mammalian celllines or stably expressed in Chinese hamster ovary(CHO)cell line.Cell proliferation was assessed by ~3H thymidineuptake.Apoptosis was examined by Hoechst 33258staining,flow cytometry and DNA fragmentation analysis.RESULTS:Reduced proliferation was readily observed inthe E2-661 expressing cells.These cells manifested thetypical features of apoptosis,including cell shrinkage,chromatin condensation and hypodiploid genomic DNAcontent.Similar apoptotic cell death was observed in anE2-661 stably expressing cell line.CONCLUSION:HCV E2 can induce apoptosis in culturedmammalian cells.
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页码：2972 / 2978
页数：7
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