Lipopeptide Antibiotics Produced by the Engineered Strain Bacillus subtilis GEB3 and Detection of Its Bioactivity

被引：0
|
作者：
Huong Pham
Joachim Vater
机构：
[1] Berlin Technical University
[2] D-10587
[3] Germany
[4] Institute of Biochemistry and Molecular Biology
来源：
Journal of Integrative Agriculture | 2004年 / 03期
关键词：
Engineered strain GEB3; Matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF-MS); LC-MS; Lipopeptide antibiotics; Surfactin; Plant pathogenic fungi; Inhibition effect;
D O I：
暂无
中图分类号：
Q939.9 [应用微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lipopeptide antibiotic surfactin. The analysisby LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chainlengths of 13,14 and 15 carbon atoms. The bioactivity detection of surfactin indicatedthat the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctoniasolani and Pyricularia oryzae.
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页码：33 / 38
页数：6
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