Diversity Analysis of Endophytic Fungi in Mentha Using Illumina MiSeq High-throughput Sequencing Technology

[Objective] This study aimed to investigate the diversity of endophytic fungi in Mentha. [Methods] By using Illumina MiS eq high-throughput sequencing technology,the 18 S rD NA-V4 region of endophytic fungi in Mentha was sequenced. Qiime and Mothur software packages were used to sort and count the number of sequences and operational taxonomic units( OTUs) and analyze the species abundance and distribution,Alpha diversity and differences of species abundance among samples. [Results]The number of effective sequences and OTUs for each sample was 64 179 and 15,respectively. The rarefaction curve showed that the sequencing deepness was large enough. The number of OTUs had almost reached a saturation point. In Mentha( BH) samples,Chao1 index and Shannon-Wiener index were 15. 0 and 0. 242,respectively. Endophytic fungi in Mentha mainly belonged to the genera Davidiella( 11. 72%) and Pleurotus( 61. 86%),but the classification status of 26. 42% of fungi had not been identified. Pleurotus was the dominant genus of endophytic fungi in Mentha. [Conclusions]Illumina MiS eq high-throughput sequencing technology provides more accurate and scientific data resources for the study of endophytic fungi in Mentha.