Studies on Anti-inflammatory Effects and Mechanism of Phellodendri Chinensis Cortex

[Objective] To observe the anti-inflammatory effects of alcohol extract of Phellodendri Chinensis Corte and explore its mechanism.[Method]ICR mice were divided into 5 groups randomly,namely the model group,dexameathone group（ 0. 01 g/kg）,alcohol extraction of Phellodendri Chinensis Corte groups（ 9. 0,4. 5 and 3. 0 g / kg）. Each drug was administrated to the mice orally once a day for 15 days. After30 min of the last administration,its anti-inflammatory effect was observed by the method of acetic acid-induced permeability accentuation. SD rats were divided into 5 groups randomly,namely the model group,dexameathone group（ 0. 01 g / kg）,alcohol extraction of Phellodendri Chinensis Corte groups（ 9. 0,4. 5 and 3. 0 g / kg）. Each drug was administrated to the rats orally once a day for 15 days. After 30 min of the last administration,the rat model of carrageenan-induced acute pleurisy was also selected to observe the anti-inflamatory effect. The anti-inflammatory mechanism was investigated by detecting the contents of prostaglandins（ PGE;）,tumor necrosis factor-alpha（ TNF-α）,and interleukin-1β（ IL-1β） in the exudates,and the contents of TNF-α,IL-1β,and malondialdehyde（ MDA） in the lung tissue were also detected.[Result]Compared with the model group,the alcohol extract of Phellodendri Chinensis Corte groups（ 9. 0 and 4. 5 g/kg） had significant inhibitory effects on the acetic acid-induced permeability accentuation in the mice,while the alcohol extract of Phellodendri Chinensis Corte groups（ 9. 0 and 4. 5g / kg） significantly reduced the levels of PGE;,TNF-α and IL-1β in the exudates（ P <0. 05）,while the high-dose alcohol extract of Phellodendri Chinensis Corte group（ 9. 0 g / kg） also reduced the contents of TNF-α,IL-1β and MDA in the lung tissue of the carrageenan treated rats（ P < 0. 01）,and the moderate-dose alcohol extract of Phellodendri Chinensis Corte group（ 4. 5 g / kg） decreased the content of IL-1β in the lung tissue（ P < 0. 01）. [Conclusion]The alcohol extraction of Phellodendri Chinensis Corte possesses significant anti-inflammatory effects,which can be related to the inhibition of generation of the inflammatory mediators and its antioxidation.