Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells

被引：0
|
作者：
栾荣华
贾国良
李伟
贾战生
连建奇
机构：
[1] Xi’an 710038
[2] Fourth Military Medical University
[3] China
[4] Department of Infectious Diseases
[5] Xi’an 710033
[6] Tangdu Hospital
[7] Xijing Hospital
[8] Department of Cardiovasology
来源：
Journal of Medical Colleges of PLA | 2003年 / 02期
基金：
中国国家自然科学基金;
关键词：
c-myc oncogene; ribozyme; vascular smooth muscle cells; gene therapy;
D O I：
暂无
中图分类号：
R341 [];
学科分类号：
1001 ;
摘要：
Ojective: To investigate the effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on theproliferation of vascular smooth muscle cells (VSMCs). Methods: Based on the computer analysis of the secondarystructure of c-myc mRNA, nt 2029 in rat c-myc oncogene was selected as a cleaving site for hammerhead ribozyme andthe ribozyme was designed. With automatic DNA synthesizer, the two complementary DNA strands of the ribozyme weresynthesized. The ribozyme gene was cloned into pGEM3Zf(+)vector and subcloned into eukaryotic expression pcD-NA3 vector. The recombinant pcDNA-Rz was transfected into the cultured rat VSMCs by lipofectAMINE mediated DNA
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页码：90 / 94
页数：5
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