Hp感染对胃黏膜FOXP3和TGF-β1及IL-10表达的影响

被引：3
|
作者：
赵振祥
高瑞红
姚红
郝素珍
机构：
[1] 山西医科大学细胞生理学省部共建教育部重点实验室
[2] 太原市疾病预防控制中心
[3] 山西医科大学微生物学与免疫学教研室
来源：
中国热带医学 | 2014年 / 11期
关键词：
幽门螺杆菌; 叉头/翼状螺旋转录因子; 转化生长因子-β1; 白介素-10;
D O I：
10.13604/j.cnki.46-1064/r.2014.11.008
中图分类号：
R573 [胃疾病];
学科分类号：
1002 ; 100201 ;
摘要：
目的研究Hp感染对胃黏膜FOXP3、TGF-β1、IL-10表达的影响。方法采用原位杂交和ELISA法检测Hp感染与未感染患者胃黏膜活检组织中FOXP3m RNA、TGF-β1m RNA的表达和IL-10的含量;用ELISA法检测胃黏膜上皮细胞GES-1与Hp共培养后的TGF-β1、IL-10含量。结果 (1)Hp感染阳性患者胃黏膜组织中FOXP3m RNA、TGF-β1m RNA的阳性表达率及IL-10的含量较Hp感染阴性患者明显增高(分别为P<0.05、P<0.05和P<0.01);(2)Hp感染阳性患者胃黏膜组织FOXP3m RNA的表达与TGF-β1m RNA和IL-10的表达呈正相关(分别为P<0.05和P<0.01);(3)胃黏膜上皮细胞GES-1与Hp共培养1h后,TGF-β1及IL-10的表达量均较阴性对照组明显增高(P<0.05)。结论 Hp感染可上调胃黏膜局部CD4+CD25+FOXP3+Treg细胞,诱导胃黏膜细胞分泌TGF-β和IL-10,从而抑制胃粘膜局部免疫,促进Hp的免疫逃逸和持续性感染。
引用
收藏
页码：1317 / 1320
页数：4
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