Extraction and Purification of Dihydromyricetin in Ampelopsis grossedentata

[Objective] To optimize the extraction and purification technology of dihydromyricetin from Ampelopsis grossedentata.[Method] Dihydromyricetin was extracted from Ampelopsis grossedentata with water as extracting agent,then purified and determined,the extraction conditions were optimized through single factor test and orthogonal experiment.[Result] The optimum extraction conditions were:extracting temperature 90 ℃,solid-liquid ratio 1:10(g:ml) and extracting time 60 min,under which the yield of dihydromyricetin was the largest,the content of total flavonoids increased from 68.51% to 91.0% after 5 times re-crystallization.[Conclusion] The extraction and purification technology was simple and affordable with convenient source of materials,therefore,it had broad prospect in producing dihydromyricetin as antioxidant.