Content Detection of Total Flavonoids in Different Parts of Rumex acetosa L.

[Objective] To establish the detection method of total flavonoids contents in Rumex acetosa L. by UV spectrophotometry,and to detect the total flavonoids contents in different parts of R. acetosa. [Methods] Effects of different factors on the extraction rate of total flavonoids from R. acetosa were researched by single factor test,which were extraction solvent,extraction method,extraction time,solid-liquid ratio and volume fraction of methanol. The optimal extraction technology of total flavonoids from R. acetosa was screened. Contents of total flavonoids were detected in different parts of R. acetosa.[Results] The optimal extraction technology of total flavonoids from R. acetosa was as follows: 70% methanol,1∶ 40 solid-liquid ratio,and hot reflux extraction for 60 min. Contents of total flavonoids in R. acetosa were the maximum in flowers and fruits.[Conclusions]The detection method for total flavonoids in R. acetosa was simple and accurate. Except the roots of R. acetosa,flowers and fruits also had high contents of total flavonoids,which could be used as the medicinal parts for the extraction of total flavonoids.