Simultaneous Detection of Four Index Components in Banxia Xiexin Decoction by RP-HPLC

[Objective]To establish the simultaneous detection method for four index components in Banxia Xiexin Decoction by RP-HPLC,which were baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid.[Methods]Diamonsil C18(2)chromatographic column(4.6 mm×250 mm,5μm)was adopted.Mobile phase was methanol(A)-0.5%phosphoric acid aqueous solution(B)with gradient elution;flow rate was 1.0 mL/min;column temperature was 30℃;detection wavelength was 230 nm,and injection volume was 20μL.[Results]The detection limits of baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid were 0.031,0.025,0.004 and 0.007μg,respectively.And the baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid all reached good isolation.Their linear ranges were0.330-16.50μg(r=0.999 5),0.347-17.37μg(r=0.999 0),0.035-1.74μg(r=0.998 4)and 0.027-1.330μg(r=0.999 3);and the average recovery rates were 101.4%(RSD=1.36%),98.5%(RSD=1.07%),96.9%(RSD=0.84%)and 99.0%(RSD=1.79%).[Conclusions]This method was simple,stable,precise and practical,and could be used for the simultaneous detection of four index components in Banxia Xiexin Decoction.