基于RNA-seq的db/db小鼠大脑皮质的转录组学分析

被引：1
|
作者：
郭宝璐
白涛
郭杰
刘萌萌
杨彩红
章毅
范彦英
机构：
[1] 山西医科大学基础医学院药理教研室
来源：
基础医学与临床 | 2020年 / 40卷 / 01期
关键词：
转录组测序; 2型糖尿病; 大脑皮质; 脑功能障碍;
D O I：
10.16352/j.issn.1001-6325.2020.01.005
中图分类号：
R587.2 [糖尿病性昏迷及其他并发症];
学科分类号：
摘要：
目的通过对db/db和野生型(WT)小鼠大脑皮质组织全转录组学分析,探索参与调节2型糖尿病诱导的脑功能障碍的差异表达基因(DEGs)及相关通路和网络。方法取雄性野生型WT和db/db小鼠各9只,在第8和24周检测小鼠的体质量和血糖,之后收集动物大脑皮质进行全转录组测序(RNA-seq),并进行DEGs,GO、KEGG及蛋白互作网络分析。结果与WT组相比,db/db组大脑皮质发生变化的306个转录本中有178个表达上调,128个表达下调。DEGs中,43个上调(如Clcnka和Trim17),59个下调(如Arih1和Nectin-3)。蛋白互作网络图中的13个枢纽基因均下调,且大多属于线粒体编码家族。同时,db/db小鼠在多项GO富集类别中具有显著差异,如细胞过程、细胞部分等。此外,KEGG功能富集结果显示DEGs在代谢、帕金森病(PD)、阿尔茨海默病(AD)等相关通路中高度富集,且这些富集通路中的DEGs主要影响了线粒体氧化磷酸化过程。结论揭示了2型糖尿病与中枢神经系统损伤之间的关系及潜在的相关基因、通路及网络。
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页码：16 / 23
页数：8
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