Phagocytosis of IgA Immune Complexes by Human U937 Cells

被引:0
|
作者
郭彩云
崔薇
张伟
机构
[1] Beijing 100005
[2] China
[3] Chinese Academy of Medical Sciences and Peking Union Medical College
[4] Institute of Basic Medical Sciences
[5] Peking Union Hospital
关键词
U937 cell FcR Immune complex IgA;
D O I
暂无
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.
引用
收藏
页码:69 / 73
页数:5
相关论文
共 50 条
  • [1] Oxidized LDL immune complexes induce release of sphingosine kinase in human U937 monocytic cells
    Hammad, SM
    Taha, TA
    Nareika, A
    Johnson, KR
    Lopes-Virella, MF
    Obeid, LM
    PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2006, 79 (1-2) : 126 - 140
  • [2] Actin in promonocytic U937 cells during differentiation and phagocytosis.
    Chaly, N
    Ferguson, K
    Meek, E
    Labow, R
    MOLECULAR BIOLOGY OF THE CELL, 1998, 9 : 15A - 15A
  • [3] Autophagy enhanced phagocytosis of apoptotic cells by oridonin-treated human histocytic lymphoma U937 cells
    Zang, Linghe
    Xu, Qian
    Ye, Yuanchao
    Li, Xin
    Liu, Yanqiu
    Tashiro, Shin-ichi
    Onodera, Satoshi
    Ikejima, Takashi
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2012, 518 (01) : 31 - 41
  • [4] IgG-FcR-mediated activation of human monocytes and U937 cells: Specific release of cytokines and maturation of U937 cells
    Micouin, A
    Bauvois, B
    BRITISH JOURNAL OF HAEMATOLOGY, 1996, 93 : 800 - 800
  • [5] BINDING OF P-32 LABELED HUMAN RECOMBINANT IMMUNE INTERFERON TO U937 CELLS
    RASHIDBAIGI, A
    KUNG, HF
    PESTKA, S
    METHODS IN ENZYMOLOGY, 1986, 119 : 315 - 321
  • [6] Progesterone metabolism in human leukemic monoblast U937 cells
    Suzuki, T
    Murry, BA
    Darnel, AD
    Sasano, H
    ENDOCRINE JOURNAL, 2002, 49 (05) : 539 - 546
  • [7] Induction of apoptosis in human leukemic U937 cells by tetrandrine
    Lai, YL
    Chen, YJ
    Wu, TY
    Wang, SY
    Chang, KH
    Chung, CH
    Chen, ML
    ANTI-CANCER DRUGS, 1998, 9 (01) : 77 - 81
  • [8] REGULATION OF PHOSPHATIDYLCHOLINE SYNTHESIS IN HUMAN LEUKEMIC U937 CELLS
    CHU, AJ
    FASEB JOURNAL, 1993, 7 (07): : A1256 - A1256
  • [9] Octylcaffeate induced apoptosis in human leukemia U937 cells
    Ujibe, M
    Kanno, S
    Osanai, Y
    Koiwai, K
    Ohtake, T
    Kimura, K
    Uwai, K
    Takeshita, M
    Ishikawa, M
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2005, 28 (12) : 2338 - 2341
  • [10] INDUCTION OF COLLAGENASE IN U937 CELLS
    HERSH, CL
    YEH, RK
    CALLAWAY, JE
    GARCIA, J
    GILMOREHEBERT, M
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1986, : 262 - 262