Effects of Curcumin on Invasion and Metastasis in the Human Cervical Cancer Cells Caski

Objective:To explore the effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski.Methods:Caski cells were treated with 10,25,50μmol/L curcumin for 24,48,72 h.Proliferation of Caski cells was measured with MTT assay.When treated with 50μmol/L curcumin for 72 h,the expressions of MMP-2,MT1-MMP and NF-κB of cells were detected by Western-blot,and invasion and metastasis of Caski cells were evaluated with transwell chamber.Results:After being treated with 10μmol/L,25μmol/L,50μmol/L curcumin for 24,48 and 72 h,the proliferation of Caski cells was inhibited in a dose-and time-dependent manner.The expression of MMP-2,MT1-MMP and NF-κB were decreased when being treated with 50μmol/L curcumin for 72 h.After treatment with 50μmol/L curcumin,in invasion assay,the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with control group(P<0.05).Meanwhile,in migration assay,the number of cells in curcumin treated group to migrate to filter was also decreased compared with control group(P<0.05).Conclusion:Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski.Inhibiting the expression of MMP-2,MT1-MMP and NF-κB was probably one of its molecular mechanisms.