Dietary synbiotic supplementation improves the growth performance,body antioxidant pool,serum biochemistry,meat quality,and lipid oxidative stability in broiler chickens

The present study investigated the effects of Lactobacillus acidophilus(LBA) and mannan-oligosaccharides(MOS) supplementation on the production performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chicken.A total of 252 commercial broiler chickens at 1 d old of uniform body weight were randomly allocated to 6 maize-soybean-based dietary treatments:T1(control diet),T2(antibiotic bacitracin methylene di-salicylate [BMD] at 20 mg/kg diet),T3(MOS at 0.1%+LBA at 106CFU/g feed),T4(MOS at 0.1%+LBA at 107CFU/g feed),T5(MOS at 0.2%+LBA at106 CFU/g feed),and T6(MOS at 0.2%+LBA at 107CFU/g feed).Each treatment was assigned to 6 replicates of 7 birds.The samples for meat quality and serum biochemistry analysis were taken from 12 birds per treatment(2 birds/replicate).The results revealed better(P <0.01) growth performance and production efficiency of birds fed either T5or T6diet compared to control or BMD supplemented diet and BMD-supplemented birds superseded the control birds.Higher(P <0.01) serum and liver antioxidant enzyme activities,meat antioxidant capacity(2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid[ABTS] and 1,1-diphenyl-2-picrylhydrazyl [DPPH] assays],serum total protein,high-density lipoproteins(HDL) cholesterol(P <0.05),and globulin levels(P <0.01) were observed in birds fed either T5or T6diet compared to control or BMD supplemented birds,whereas,lower lipid oxidation(P <0.01),cardiac risk ratio,atherogenic coefficient,atherogenic index of plasma,serum glucose,triglyceride,total cholesterol levels(P <0.01),and serum albumin-to-globulin ratio(P <0.05) were observed in the chickens.The pH of meat from birds fed T4,T5or T6diet was lower(P <0.01) compared to control and other treatments.The extract release volume(ERV),water holding capacity(WHC),and protein content of meat were higher(P <0.05) in birds fed either T5or T6diet compared to control or BMD supplemented birds.Thus,it was concluded that the supplementation of 0.2% MOS along with LBA at 106CFU/g is optimum for better growth performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chickens.