Based on the measurement of L-band ground-based microwave radiometer(ELBARA-III type) in the Qinghai-Tibet Plateau and the τ-ω radiative transfer model, this research evaluated the effects of four soil dielectric models, i. e., WangSchmugge, Mironov, Dobson, and Four-phase, on the L-band microwave brightness temperature simulation and soil moisture retrieval. The results show that with the same vegetation and roughness parameterization scheme, the four soil dielectric models display obvious differences in microwave brightness temperature simulation. When the soil moisture is less than 0.23 m;/m;, the simulated microwave brightness temperature in Wang-Schmugge model is significantly different from that of the other three models, with maximum differences of horizontal polarization and vertical polarization reaching 8.0 K and 4.4 K, respectively; when the soil moisture is greater than 0.23 m;/m;, the simulated microwave brightness temperature of Four-phase significantly exceeds that of the other three models; when the soil moisture is saturated, maximum differences in simulated microwave brightness temperature with horizontal polarization and vertical polarization are 6.1 K and 4.8 K respectively, and the four soil dielectric models are more variable in the microwave brightness temperature simulation with horizontal polarization than that with vertical polarization. As for the soil moisture retrieval based on the four dielectric models, the comparison study shows that, under the condition of horizontal polarization, Wang-Schmugge model can reduce the degree of retrieved soil moisture underestimating the observed soil moisture more effectively than other parameterization schemes, while under the condition of vertical polarization, the Mironov model can reduce the degree of retrieved soil moisture overestimating the observed soil moisture. Finally, based on the Wang-Schmugge model and Feng Yun-3C observation data, the spatial distribution of soil moisture in the study area is retrieved.