Heparanase antisense oligodeoxynucleotide inhibits angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice

被引：0
|
作者：
张友磊 [1 ]
傅志仁 [2 ]
丁国善 [2 ]
张军 [3 ]
沈茜 [3 ]
王义 [1 ]
机构：
[1] Eastern Hepatobiliary Surgery Hospital,Second Military Medical University
[2] Liver Transplantation Center,Changzheng Hospital,Second Military Medical University
[3] Central Laboratory,Changhai Hospital,Second Military Medical University
来源：
Journal of Medical Colleges of PLA | 2007年 / 03期
关键词：
heparanase; antisense oligodeoxynucleotide; mammary carcinoma; angiogenesis; metastasis;
D O I：
暂无
中图分类号：
R737.9 [乳腺肿瘤];
学科分类号：
摘要：
Objective:To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide (AS-ODN) on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods:The AS-ODN complementary to the start codon region of heparanase mRNA and its control, scrambled nonsense oligodeoxynucleotide (NS-ODN) were designed and synthesized.A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were estab- lished in nude mice and were treated with ODNs.The heparanase expression in tumor was evaluated by RT-PCR and Western blot.The microvessel density (MVD) was measured by immunohistochemistry for factorⅧ.The tumor volume was calculated and lung metastatic nodules were counted.Results:The hep- aranase expression,MVD,tumor volume and lung metastatic nodules in AS-ODN treated group were sig- nificantly decreased compared with that in NS-ODN treated group and that in PBS group (P＜0.01).Con- clusion:Heparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.
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页码：143 / 148
页数：6
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