Assembly and Immunogenicity of Human Papillomavirus Type 16 Major Capsid Protein(HPV16 L1) in Pichia pastoris

被引：0
|
作者：
LIU Da-wei
机构：
来源：
Chemical Research in Chinese Universities | 2007年 / 02期
基金：
中国国家自然科学基金;
关键词：
Human papillomavirus; Major capsid protein; Recombinant Pichia pastoris; Virus-like particles;
D O I：
暂无
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven by a strong AOX1 promoter. HPV16L1 gene was cloned into vector pPICZ%α%B. HPV16 L1 protein expression induced by methanol was screened by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting. The results indicate that the HPV16 L1 protein is secreted by the recombinant P. pastoris, and the purified HPV16 L1 protein can self-assemble into virus-like particles(VLPs), which show a good immunogenicity and induces high-titer antibody in mice.
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页码：200 / 203
页数：4
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