Effect of Buchanania lanzan Spreng.bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice

Objective:To elucidate the effect of ethanolic extract of Buchanania lanzan Spreng.(B.lanan) bark against cyclophosphamide induced genotoxicity and oxidative stress in mice.Methods: The prevalence of micronuclei in bone marrow,the extent of lipid peroxidation,reduced glutathione and the status of the antioxidant enzymes,superoxide dismutase and catalase in liver of mice were used as intermediate biomarkers for chemoprolection.Lipid peroxidation and associated compromised antioxidant defenses in cyclophosphamide treated mice were observed in the liver.Results:Pre-treatment with B.lanzan 250,500 and 1 000 mg/ kg,p.o.,daily for 7 days significantly reduced the chromosomal damage and lipid peroxidation with concomitant changes in antioxidants and detoxification systems.Conclusions:These results point out the presence of chemopreventive phytoconstituents in the crude extract offering protection against cyclophosphamide induced genotoxicity and oxidative stress in mice.