鼻咽癌DLC1基因的表达研究

被引：8
|
作者：
彭宏
赵彤
姚开泰
机构：
[1] 第一军医大学肿瘤研究所 510515广州
[2] 第一军医大学南方医院耳鼻咽喉科
[3] 第一军医大学肿瘤研究所
来源：
中华耳鼻咽喉头颈外科杂志 | 2002年 / 06期
基金：
广东省自然科学基金;
关键词：
鼻咽肿瘤; 基因; 抑制; 肿瘤; 肿瘤细胞; 培养的;
D O I：
暂无
中图分类号：
R739.63 [咽肿瘤];
学科分类号：
100214 ;
摘要：
目的　探讨DLC1(deletedinlungcancer 1)基因在鼻咽癌发生中的作用。方法　应用逆转录 -聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)方法检测 4种鼻咽癌细胞株、3 2例鼻咽癌组织和 16例正常鼻咽部组织DLC1基因的表达 ,对发现的异常转录进行DNA测序分析。结果　 4种鼻咽癌细胞株均不表达DLC1基因 ,鼻咽癌组织DLC1基因表达明显低于正常鼻咽部组织 (P <0 0 0 1) ;局部T3、T4期鼻咽癌DLC1基因表达明显低于T1、T2期 (P =0 0 1) ;然而 ,DLC1基因表达与鼻咽癌患者的年龄、性别、颈部淋巴结转移 ,全身远处转移、TNM临床分期和血清EB病毒VCA IgA抗体滴度均无相关性。 3 2例鼻咽癌组织中有 7例发生DLC1基因第 8外显子的异常剪接 ,而 16例正常鼻咽部组织中仅 1例在表达正常DLC1转录的同时伴有该异常转录的表达。结论　DLC1基因的表达异常与鼻咽癌的发生可能密切相关 ,其低表达可能与鼻咽癌的局部侵袭力有关。DLC1基因第 8外显子的异常剪接与DLC1基因的功能异常有关
引用
收藏
页码：57 / 60+105
页数：5
相关论文
共 14 条
  • [1] Sequence analysis of a 685-kb genomic region on chromosome 3p22-p21. 3 that is homozygously deleted in a lung carcinoma cell line. Ishikawa S,Kai M,Tamari M,et al. DNA Research . 1997
  • [2] Microsatellite allelotyping of chinese nasopharyngeal carcinomas. Lung ML,Choi CV,Kong H,et al. Anticancer Research . 2001
  • [3] High resolution allelotype of microdissected primary nasopharyngeal carcinoma. Lo KW,Teo PM,Hui AB,et al. Cancer Research . 2000
  • [4] Functional evidence for a nasopharyngeal carcinoma tumor suppressor gene that maps at chromosome 3p21. 3. Cheng Y,Poulos NE,Lung ML,et al. Proceedings of the National Academy of Sciences of the United States of America . 1998
  • [5] Molecular cloning of a candidate tumor suppressor gene, DLC1, from chromosome 3p21. 3. Daigo Y,Nishiwaki T,Kawasooe T,et al. Cancer Research . 1999
  • [6] Combined LOH/CGH analysis proves the existence of interstitial 3p deletions in renal cell carcinoma. Alimov A,Kost-Alimova M,Liu J,et al. Oncegene . 2000
  • [7] High resolution chromosome 3p allelotyping of human lung cancer and preneoplastic/preinvasive bronchial epithelium reveals multiple, discontinuous sites of 3p allele loss and three regions of frequent breakpoints. Wistuba II,Behrens C,Virmani AK,et al. Cancer Research . 2000
  • [8] Mutagen sensitivity and environmental exposures as contributing causes of chromosome 3p losses in head and neck cancers. Schantz SP,Huang Q,Shah K,et al. Carcinogenesis . 2000
  • [9] High-resolution chromosome 3p allelotyping of breast carcinomas and precursor lesions demonstrates frequent loss of heterozygosity and a discontinuous pattern of allele loss. Maitra A,Wistuba II,Washington C,et al. American Journal of Pathology . 2001
  • [10] Analysis of intratumoral heterogeneity of chromosome 3p deletions and genetic evidence of polyclonal origin of cervical squamous carcinoma. Guo Z,Wu F,Asplund A,et al. Modern Pathology . 2001
12