Attraction of bruchid beetles Callosobruchus chinensis(L.)(Coleoptera: Bruchidae) to host plant volatiles

Host-plant volatiles play an important role as cues for herbivores in search of resources, mates and oviposition sites in complex environments.Plant volatile-based attractants can be developed for pest monitoring and control.Previously, we indicated that mated female adults of Callosobruchus chinensis showed choice preference behavior toward 2-hexenal and benzaldehyde.Our objective here was to investigate the synergistic effect of host-derived attractive volatiles in attracting C.chinensis under laboratory and field conditions in Shanxi Province, China.We hypothesized that the ratio and concentration of volatiles derived from Vigna radiata play critical roles for C.chinensis in locating this host.Therefore, we collected and identified the volatiles of mungbean by using headspace collection and GC-MS.The effectiveness of different ratios and concentrations of two compounds(2-hexenal and benzaldehyde) that elicit C.chinensis searching behavior were examined in Y-tube olfactometer assays.The combination of 300 μgμL2-hexenal and 180 μgμLbenzaldehyde loadings exhibited a synergistic effect on attracting C.chinensis(82.35%).Compared to control traps, the adults were significantly attracted to traps baited with blends, and more attraction to females than males was found for blend traps in the field experiments.Our results suggest that blends of this specific concentration and ratio of benzaldehyde and 2-hexenal can be used in traps as attractants for C.chinensis monitoring and control in the field.