Effects of ginkgo biloba extract on the expressions of IL-1β,TNF-α,IL-10 and IL-10R in heart of atherosclerotic rats

被引:0
|
作者
焦亚斌
芮耀诚
李铁军
杨鹏远
邱彦
机构
[1] China
[2] Shanghai 200433
[3] Department of Pharmacology School of Pharmacy Second Military Medical University
基金
中国国家自然科学基金;
关键词
atherosclerosis; cytokine; interleukin-1; tumor necrosis factor; interleukin-10; Ginkgo biloba; rats;
D O I
暂无
中图分类号
R543 [血管疾病];
学科分类号
1002 ; 100201 ;
摘要
Objective: To study whether the anti-AS effect of ginkgo biloba extract (GbE) was related with inhibitory effects on the expression of IL-1β, TNF-αand up-regulation of IL-10 and IL-10R in the heart of atherosclerotic (AS) rats. Methods : The experimental model of AS rats were established by in-traperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups: control,AS and GbE. GbE (100 mg/kg) was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results:The protein and mR-NA expressions of IL-1β,TNF-α,IL-10 and IL-10R were markedly higher in AS group than those in control group (P<0. 01). The protein and mRNA expressions of IL-1βand TNF-αwere markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group (P<0. 01). Conclusion: GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cy-tokine IL-10,IL-10R that may be partially responsible for its anti-AS effects.
引用
收藏
页码:347 / 351
页数:5
相关论文
共 50 条
  • [1] IL-10 RECEPTOR (IL-10R) TRANSFECTANTS RESPONSES TO VIRAL IL-10 - EVIDENCE FOR ADDITIONAL IL-10R COMPONENTS
    XUAMANO, JC
    LIU, Y
    WEI, SHY
    HO, ASY
    HSU, AKH
    MOORE, KW
    [J]. FASEB JOURNAL, 1995, 9 (04): : A772 - A772
  • [2] Serum IL-1β, IL-6, IL-10 and TNF-α Levels in Thyroidectomized Rats
    Kandir, Sinan
    Keskin, Ercan
    [J]. KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2016, 22 (02) : 297 - 300
  • [3] Comparative quantification of IL-1β, IL-10, IL-10r, TNFα and IL-7 mRNA levels in UV-irradiated human skin in vivo
    Brink, N
    Szamel, M
    Young, AR
    Wittern, KP
    Bergemann, J
    [J]. INFLAMMATION RESEARCH, 2000, 49 (06) : 290 - 296
  • [4] Comparative quantification of IL-1β, IL-10, IL-10r, TNFα and IL-7 mRNA levels in UV-irradiated human skin in vivo
    N. Brink
    M. Szamel
    A.R. Young
    K.P. Wittern
    J. Bergemann
    [J]. Inflammation Research, 2000, 49 : 290 - 296
  • [5] IL-1α, IL-1β, IL-Rα and IL-10 in seronegative spondyloarthropathies.
    Peddle, L
    Snelgrove, T
    Saile, M
    Butt, C
    Hamilton, S
    Rahman, P
    [J]. ARTHRITIS AND RHEUMATISM, 2004, 50 (09): : S614 - S614
  • [6] Effects of asymmetric dimethylarginine on inflammatory cytokines (TNF-α, IL-6 and IL-10) in rats
    Avci, Bahattin
    Alacam, Hasan
    Dilek, Ahmet
    Kozan, Ahmet
    [J]. TOXICOLOGY AND INDUSTRIAL HEALTH, 2015, 31 (03) : 268 - 273
  • [7] Changes in TNF-α, IL-6, IL-10 and VEGF in rats with ARDS and the effects of dexamethasone
    Qin, Mengting
    Qiu, Zhongpeng
    [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2019, 17 (01) : 383 - 387
  • [8] Induced heteroduplex genotyping of TNF-α, IL-1β, IL-6 and IL-10 polymorphisms associated with transcriptional regulation
    Morse, HR
    Olomolaiye, OO
    Wood, NAP
    Keen, LJ
    Bidwell, JL
    [J]. CYTOKINE, 1999, 11 (10) : 789 - 795
  • [9] Cytokines (TNF-α, IL-1 β, IL-2, IL-10) as serum tumor markers in multiple myeloma.
    Grigoraki, V
    Regli, A
    Gortzolidis, G
    Zomas, A
    Michalis, E
    Galanopoulos, A
    Marinakis, T
    Dimopoulos, J
    Papadimitriou, S
    Alexandropoulos, K
    Matsis, K
    Anagnostopoulos, NI
    [J]. BLOOD, 2000, 96 (11) : 275B - 275B
  • [10] Evaluation of corticosterone and IL-1β, IL-6, IL-10 and TNF-α expression after 670-nm laser photobiomodulation in rats
    Marques Lima, Anuska Aparecida
    Spinola, Lais Gomes
    Baccan, Gisele
    Correia, Kariza
    Oliva, Marcos
    Vasconcelos, Juliana F.
    Soares, Milena B. P.
    Reis, Silvia Regina
    Medrado, Alena Peixoto
    [J]. LASERS IN MEDICAL SCIENCE, 2014, 29 (02) : 709 - 715