Activation of Signal Transducer and Activator of Transcription 5 (STAT5) in Splenocyte Proliferation of Asthma Mice Induced by Ovalbumin

To investigate the role of signal transducer and transcriptional activator 5（STAT5）activated in ovalbumin（OVA）-induced splenocyte proliferation of asthma mice,an asthma mouse model was set up by intraperitonealinjection and aspiration of OVA with nebulizer.The proliferation of splenocytes isolated from the asthma micewas detected by [;H]thymidine incorporation.The phosphorytation of STAT5 was examined by Westernblotting and STAT5-DNA binding was measured by electrophoretic mobility shift assay（EMSA）.OVA couldpronouncedly induce the splenocyte proliferation of asthma mice in a dose-dependent manner compared withcontrol groups.Phosphorylation of STAT5 and STAT5-DNA binding were observed in splenocytes fromasthma mice induced by OVA at 1 h and 3 h.These results indicated that STAT5 signal pathway played animportant role in lymphocyte proliferation of asthma mice induced by OVA.Cellular & Molecular Immunology.2004;1（6）:471-474.