CHARACTERIZATION OF VP1 AS IMMUNODOMINANT ANTIGEN OF ENTEROVIRUS TYPE 70 ABID ANTIGENIC ANALYSIS OF VIRUS STRAINS BY MONOCLONAL ANTIBODIES

The neutralizing antigens of eliterovirus type70 (E70) are characterized immunochemically. It was found that all of the 7 neutralizing McAbs tested could cambine with both naturally-occurring topcomponents (NTC) and virions in immunodot test, five of them could combine with VP1 in the immnuno-blot system, and patient sera and antibodies from immune and hyperimmune animals could only cambine with VP1. Furthermore, 10 McAbs (including the mentioned 7) were used to analyse the antigenic differences of 8 E70 strains isolated in China in 1985 and the prototype virus J670/71. The results indicate that the 10 McAbs can neutralize all 9 viruses almost equally. The data indicate that VP1 is immuno-dominant and stable enough to keep neutralizing antigenicity during treatment in the immunoblot system.