MACC1在结肠癌中的表达及与上皮间质转化的关系

被引：4

作者：

刘浩峰

陈铁

江晓晖

孙跃明

机构：

[1] 南通市肿瘤医院普外科

来源：

交通医学 | 2016年 / 30卷 / 05期

关键词：

MACC1; 结肠癌; 上皮-间质转化; HCT116;

D O I：

暂无

中图分类号：

R735.35 [];

学科分类号：

摘要：

目的:研究MACC1在结肠癌组织中的表达及其在结肠癌EMT过程中的作用。方法:按结肠癌病理分期,将患者标本分位N组(距肿瘤5cm的正常组织)、AB组(I,II期),CD组(III,IV期),采用RT-PCR检测MACC1在各组中的表达情况;将融合绿色荧光蛋白(green fluorescent proteins,GFP)的质粒干扰具有转移能力的结肠癌细胞株HCT116,应用RT-PCR、Western blot检测下调MACC1后EMT相关蛋白(E-cadherin、Vimentin)表达情况,应用划痕实验和Transwell实验比较转染前后细胞的侵袭迁移能力。结果:RT-PCR结果显示MACC1在结肠癌组织中表达较正常黏膜组织明显增高(P<0.05);有淋巴结转移的较未发生淋巴结转移的表达增高(P<0.05);HCT116经干扰后,Ecadherin蛋白表达升高、Vimentin蛋白表达降低,侵袭转移能力降低(P<0.05)。结论 :MACC1基因在结肠腺癌组织中过表达,与结肠癌的侵袭、转移密切相关。MACC1基因促进了结肠腺癌的EMT过程,增强了肿瘤细胞的侵袭转移能力。

引用

页码：434 / 437+442 +442

页数：5

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