microRNA-29a-3p通过p70s6k/S6信号通路促进乳腺癌细胞增殖

被引：0

作者：

曹琦雪

郝苗苗

李世萍

孙明芳

刁可心

王健

米小轶

机构：

[1] 中国医科大学基础医学院病理学教研室

来源：

解剖科学进展 | 2021年 / 27卷 / 03期

关键词：

miR-29a-3p; 乳腺癌细胞; 增殖;

D O I：

10.16695/j.cnki.1006-2947.2021.03.007

中图分类号：

R737.9 [乳腺肿瘤];

学科分类号：

摘要：

目的研究microRNA-29a-3p在乳腺癌细胞系中的表达,探讨miR-29a-3p对p70s6k/S6信号通路以及对乳腺癌细胞增殖能力的影响。方法首先通过实时聚合酶链反应检测乳腺癌细胞系MCF-7、MDA-MB-231和正常乳腺细胞MCF-10A中miR-29a-3p的表达水平,选取miR-29a-3p表达较高的乳腺癌细胞MCF-7进行后续实验;通过Western blot检测转染后p70s6k/S6信号通路中关键基因的表达,并通过MTT实验明确miR-29a-3p对MCF-7细胞增殖能力的影响。结果 miR-29a-3p在乳腺癌细胞中高表达;在MCF-7细胞中转染miR-29a-3p mimic后,p70s6k和S6的磷酸化水平显著增加,p70s6k和S6的总蛋白量无明显变化,而转染miR-29a-3p inhibitor后,呈现相反结果;此外,MTT实验显示miR-29a-3p的表达与乳腺癌细胞的增殖能力呈正相关。结论 miR-29a-3p通过p70s6k/S6信号通路促进乳腺癌细胞的增殖。

引用

页码：280 / 283

页数：4

共 6 条

[1] The potential role of miR-29 in health and cancer diagnosis, prognosis, and therapy
Alizadeh, Mohsen
Safarzadeh, Ali
Beyranvand, Fatemeh
Ahmadpour, Fatemeh
Hajiasgharzadeh, Khalil
Baghbanzadeh, Amir
Baradaran, Behzad
[J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (11) : 19280 - 19297
[2] Cytoplasmic TRAF4 contributes to the activation of p70s6k signaling pathway in breast cancer
Ren, Hua-Yan
Wang, Jian
Yang, Fan
Zhang, Xiao-Li
Wang, Ai-Lian
Sun, Li-Li
Diao, Ke-Xin
Wang, En-Hua
Mi, Xiao-Yi
[J]. ONCOTARGET, 2015, 6 (06) : 4080 - 4096
[3] A review of clinical aspects of breast cancer
Libson, Shai
Lippman, Marc
[J]. INTERNATIONAL REVIEW OF PSYCHIATRY, 2014, 26 (01) : 4 - 15
[4] The role of microRNA in metastatic colorectal cancer and its significance in cancer prognosis and treatment
Tokarz, Paulina
Blasiak, Janusz
[J]. ACTA BIOCHIMICA POLONICA, 2012, 59 (04) : 467 - 474
[5] The insulin-like growth factors and insulin-signalling systems: an appealing target for breast cancer therapy?[J] . Gray S G,Stenfeldt Mathiasen I,De Meyts P.Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme . 2003 (11-1)
[6] Genetic Alterations of TRAF Proteins in Human Cancers .2 Zhu S,Jin J,Gokhale S,et al. Front Immunol . 2018

← 1 →