Neurotoxicity of prenatal alcohol exposure on medullary pre-B?tzinger complex neurons in neonatal rats

被引:1
|
作者
Ming-li Ji [1 ]
Yun-hong Wu [2 ]
Zhi-bin Qian [2 ]
机构
[1] Department of Physiology, Xinxiang Medical University
[2] Department of Functional Laboratory, Xinxiang Medical University
关键词
nerve regeneration; brain injury; prenatal alcohol exposure; pre-B?tzinger complex; respiratory depression; neonatal rats; medullary slice; medullary respiratory center; respiratory rhythmical discharge activity; respiratory neuron; 5-hydroxytryptamine 2A receptor; neural regeneration;
D O I
暂无
中图分类号
R714.2 [病理妊娠(异常妊娠)];
学科分类号
100211 ;
摘要
Prenatal alcohol exposure disrupts the development of normal fetal respiratory function, but whether it perturbs respiratory rhythmical discharge activity is unclear. Furthermore, it is unknown whether the 5-hydroxytryptamine 2A receptor(5-HT2AR) is involved in the effects of prenatal alcohol exposure. In the present study, pregnant female rats received drinking water containing alcohol at concentrations of 0%, 1%, 2%, 4%, 8% or 10%(v/v) throughout the gestation period. Slices of the medulla from 2-day-old neonatal rats were obtained to record respiratory rhythmical discharge activity. 5-HT2 AR protein and m RNA levels in the pre-B?tzinger complex of the respiratory center were measured by western blot analysis and quantitative RT-PCR, respectively. Compared with the 0% alcohol group, respiratory rhythmical discharge activity in medullary slices in the 4%, 8% and 10% alcohol groups was decreased, and the reduction was greatest in the 8% alcohol group. Respiratory rhythmical discharge activity in the 10% alcohol group was irregular. Thus, 8% was the most effective alcohol concentration at attenuating respiratory rhythmical discharge activity. These findings suggest that prenatal alcohol exposure attenuates respiratory rhythmical discharge activity in neonatal rats by downregulating 5-HT2 AR protein and m RNA levels.
引用
收藏
页码:1095 / 1100
页数:6
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