EFFECTS OF METHYLENE BLUE ON THE BLOOD PRESSUREAND CGMP OF ENDOTOXIC SHOCK RABBITS

Objective To investigate the effect of methylene blue (MB) on the blood pressure and cGMP ofendotoxic shock. Methods An experiment was performed on 16 New Zealand rabbits suffering from endotoxicshock, in which 8 were distributed to the trial group (MB infusion) and another 8 to the control group (normalsaline). The mean arterial pressure (MAP), plasma cyclic monophosphate guanylate (cGMP), and arterialnatriuretic factor (ANF) in the two groups were observed. Results in the trial group MB infusion elevated MAP(P<0.01), decreased cGMP (P<0.01) and did not change the level of ANF. In the control group, normal salineinfusion did not alter MAP, plasma cGMP and ANF level. In addition, the MAP of the trial group was foundsignificantly higher than that of the control group (P<0.01) and the plasma cGMP of the trial group significantlylower than that of the control group (P<0.01). Conclusion These data suggest that the elevation of plasmacGMP is related to hypotension and MB in vivo can effectively inhibit soluable guanylate Cyclase, thus decreaseplasma cGMP level and increase MAP of rabbits with endotoxic shock. This indicates that MB can be used as adrug for the treatment of endotoxic shock.