深绿木霉(Trichoderma atroviride)T23降解有机磷农药敌敌畏转运蛋白TaPdr2基因的克隆与功能预测分析

被引：4
|
作者：
孙佳楠 [1 ]
张泰龙 [1 ]
陈硕闻 [2 ]
陈捷 [1 ]
戴宝 [3 ]
机构：
[1] 上海交通大学农业与生物学院农业部都市农业(南方)重点开放实验室
[2] 上海大井生物工程有限公司
[3] 山东泰诺药业有限公司
来源：
上海交通大学学报(农业科学版) | 2016年 / 34卷 / 06期
关键词：
深绿木霉T23; 敌敌畏; ABC转运蛋白; TaPdr2;
D O I：
暂无
中图分类号：
S435.13 [玉米病虫害];
学科分类号：
摘要：
根据前期研究,进行了对比分析后明确了深绿木霉中7个敌敌畏耐受相关ABC转运蛋白,其中TaPdr2基因是在短时间内对敌敌畏胁迫应答最为明显的一个基因。本研究通过简并引物同源克隆,在深绿木霉T23中克隆出TaPdr2基因,并对其全长序列进行了测序;明确了其外显子序列和内含子序列;构建了TaPdr2的系统发生树,发现深绿木霉TaPdr2与木霉属其他种PDR5亚家族ABC转运蛋白有很高的同源性,通过生物信息学分析后预测TaPdr2蛋白的ATPase及部分跨膜结构域序列相对保守且具有11个跨膜结构域;通过构建TaPdr2的敲除载体pC1300qh-F,为后续研究TaPdr2蛋白对麦角甾醇和鞘脂类物质含量影响的研究奠定了基础。
引用
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页码：1 / 8
页数：8
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