EFFECTS OF HYPOXIC ENDOTHELIAL CELL CONDITIONED MEDIUM ON PROLIFERATION AND COLLAGEN SYNTHESIS OF SMOOTH MUSCLE CELLS AND INHIBITORY EFFECTS OF RADIX SALVIAE MILTIORRHIZAE

被引：0

作者：

孙宝华 ^{[1
]}

袁永辉 ^{[1
]}

张婉蓉 ^{[1
]}

车东媛 ^{[1
]}

机构：

[1] Department of Pathology School of Basic Medical Sei-ences Tongji Medical University Wuhan 430030

来源：

中华医学杂志(英文版) | 1995年 / 11期

关键词：

RSM; ECCM; EFFECTS OF HYPOXIC ENDOTHELIAL CELL CONDITIONED MEDIUM ON PROLIFERATION AND COLLAGEN SYNTHESIS OF SMOOTH MUSCLE CELLS AND INHIBITORY EFFECTS OF RADIX SALVIAE MILTIORRHIZAE; SFM;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

The effects of hypoxic endothelial cell conditioned medium (HECCM) on proliferation and collagen synthesis of cultured porcine pulmonary arterial smooth muscle cells (PASMCs) were studied by 3H-thymidine (3H-TdR) and 3H-proline incorporations, image analysis for determination of DNA content and colorimetric assay using MTT, and the inhibitory effects of radix salviae miltiorrhizae (RSM) on them were also investigated. The results showed that HECCM could induce enhancement of the enzymatic activity of mitochondria, increase of the nucleic DNA content and increases of the 3H-TdR and 3H-proline incorporations in PASMCs. The 3H-proline incorporation in PASMCs cultured in HECCM was 1.83 times as much as that cultured in normoxic endothelial cell conditioned medium (NECCM). Compared with the control, Chinese herb medicine RSM could inhibit the proliferation of PASMCs cultured in HECCM and decrease the 3H-prolinc incorporation in PASMCs cultured in both HECCM and NECCM (P< 0.001). However, RSM had no ef fects o

引用

页码：57 / 60

页数：4

共 50 条

[1] EFFECTS OF HYPOXIC ENDOTHELIAL CELL CONDITIONED MEDIUM ON PROLIFERATION AND COLLAGEN SYNTHESIS OF SMOOTH MUSCLE CELLS AND INHIBITORY EFFECTS OF RADIX SALVIAE MILTIORRHIZAE
孙宝华
袁永辉
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车东媛
[J]. Chinese Medical Journal, 1995, (11)
[2] EFFECTS OF HYPOXIC ENDOTHELIAL-CELL CONDITIONED MEDIUM ON PROLIFERATION AND COLLAGEN-SYNTHESIS OF SMOOTH-MUSCLE CELLS AND INHIBITORY EFFECTS OF RADIX SALVIAE MILTIORRHIZAE
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[4] Effects of Tetramethylpyrazine and Radix Salviae Miltiorrhizae on collagen synthesis and proliferation of cardiac fibroblasts
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[5] THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE ON THE INHIBITORY ACTIVITY OF VASCULAR SMOOTH MUSCLE CELLS TO THE PLASMINOGEN ACTIVATORS SECRETED BY ENDOTHELIAL CELLS
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[6] PREVENTIVE EFFECTS OF POLLEN TYPHAE AND RADIX SALVIAE MILTIORRHIZAE IN ATHEROGENESIS——INHIBITION ON PROLIFERATION OF AORTIC SMOOTH MUSCLE CELLS IN CULTURE
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[8] Effects of the medium conditioned by endothelial cells under hypoxic condition on the phenotype of porcine pulmonary arterial smooth muscle cells
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[9] Effects of the Medium Conditioned by Endothelial Cells under Hypoxic Condition on the Phenotype of Porcine Pulmonary Arterial Smooth Muscle Cells
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[10] Inhibitory effects of tranilast on proliferation, migration, and collagen synthesis of human vascular smooth muscle cells
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