Effects of berberine on L-and T-type calcium channels in guinea pig ventricular myocytes

被引：0
|
作者：
徐尚忠
张翼
任建英
周兆年
机构：
[1] 上海市徐汇区中心医院心血管病研究室
[2] 中国科学院上海生理研究所
[3] 河北医科大学生理学教研室
来源：
ActaPharmacologicaSinica | 1997年 / 06期
关键词：
小檗碱; 膜片箝技术; 心肌; 心脏; 抗心律失常药; 离子通道; 钙通道;
D O I：
暂无
中图分类号：
R972.2 [];
学科分类号：
1007 ;
摘要：
目的：研究小檗碱（Ｂｅｒ）对心室肌细胞钙通道的影响．方法：全细胞膜片箝技术．结果：Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使豚鼠心室肌细胞Ｌ型钙流由１４００±２４７ｐＡ分别减至９７８±２０４ｐＡ及６１７±２３ｐＡ（ｎ＝５，Ｐ＜００５），抑制效应呈浓度依赖及非频率依赖，其电流－电压曲线的峰值下降．Ｂｅｒ（１０μｍｏｌ·Ｌ－１）使Ｌ型钙流失活曲线的最大半激活电压由－２７８ｍＶ变为－３４２ｍＶ，斜率因子由９２２变为１３０３，对激活曲线无影响．Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使Ｔ型钙流峰值由加药前的１５４±８０ｐＡ降至１０１±７８ｐＡ及４８±４５ｐＡ（ｎ＝８，Ｐ＜００５）．结论：Ｂｅｒ对Ｌ和Ｔ型钙通道均有抑制作用．
引用
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页码：35 / 38
页数：4
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