Determination of S/R ratio of mephenytoin in human urine by chiral HPLC and ultraviolet detection and its comparison with gas chromatography

被引：0

作者：

黄松林 ^{[1
]}

谢红光 ^{[1
]}

王伟 ^{[1
]}

许振华 ^{[1
]}

蒋长虹 ^{[1
]}

周宏灏 ^{[1
]}

机构：

[1] 湖南医科大学遗传药理学研究所

来源：

Acta Pharmacologica Sinica | 1998年 / 06期

关键词：

美芬妥英; 细胞色素P-450CYP2C19; 高压液相色谱法; 药物遗传学; 气相色谱法;

D O I：

暂无

中图分类号：

R968,R914.1 [];

学科分类号：

100602 ; 100706 ;

摘要：

目的：建立人尿中Ｒ，Ｓ美芬妥英的液相色谱手性分离与检测方法，用于ＣＹＰ２Ｃ１９酶活性的快速测定．方法：受试者口服消旋美芬妥英后的０－８ｈ尿液标本用二氯甲烷提取后用手性色谱柱进行分离，流动相为乙腈和水（１４∶８６，体积比），其中含有０．１％的冰醋酸和０．２％的三乙胺，流速为０．９ｍＬ·ｍｉｎ－１，紫外检测波长为２０７ｎｍ．结果：在选定的色谱条件下Ｒ，Ｓ美芬妥英能很好地分离，尿中其他物质无干扰．用外标法定量，线性范围在５０－５０００μｇ·Ｌ－１，最小检出浓度为１２．５μｇ·Ｌ－１，保留时间在９ｍｉｎ内．液相色谱分析结果和气相色谱分析具有良好的一致性．结论：该法样本制备简便、分析时间短、线性范围宽、干扰少、灵敏和准确，可用于人体内美芬妥英代谢的研究和肝药酶ＣＹＰ２Ｃ１９酶活性的检测

引用

页码：49 / 51

页数：3

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