Sperm-Borne Mitochondrial Activity Influenced by Season and Age of Holstein Bulls

Sperm mitochondria are vital organelles for energy production and pre- and post-fertilization sperm functions. The potential influence of the age of the bull and season on the sperm-borne mitochondrial copy number and the transcription activity has not yet been investigated. Therefore, the expression patterns of all protein-coding mitochondrial genes were identified throughout the year along with mitochondrial copy numbers in young and old bulls' spermatozoa. For that, high-quality semen samples (n = 32) with more than 80% quality for the morphological parameters, from young (n = 4, aged 18-24 months old) and old (n = 4, aged 40-54 months old) Holstein bulls, were collected during the four seasons (n = 4 samples each animal/season). The DNA and RNA were isolated from sperm cells and subjected to the DNA copy number and expression analyses using qPCR. Furthermore, an in silico analysis using gene ontology online tools for the abundantly expressed genes was utilized. The data were statistically analyzed using Prism10 software. There was a significant reduction in the mitochondria copy number of young bulls' spermatozoa compared to their old counterparts during the summer (29 +/- 3 vs. 51 +/- 6, p < 0.001) and winter (27 +/- 3 vs. 43 +/- 7, p < 0.01) seasons. However, sperm-borne mitochondrial protein-coding genes were transcriptionally higher in young bulls throughout the year. Within the same group of bulls, unlike the old bulls, there was a significant (p < 0.05) induction in the transcription activity accompanied by a significant (p < 0.05) reduction in the mitochondrial copy numbers in the summer (29 +/- 3) and winter (27 +/- 3) compared to the spring (42 +/- 9) and autumn (36 +/- 5) seasons in young bulls. Additionally, the pathway enrichment of the top six expressed genes differed between age groups and seasons. In conclusion, under the same quality of semen, the early stages of age are associated with mitochondrial biogenesis and transcription activity dysregulation in a season-dependent manner.