A Study of the Bioactive Compounds, Antioxidant Capabilities, Antibacterial Effectiveness, and Cytotoxic Effects on Breast Cancer Cell Lines Using an Ethanolic Extract from the Aerial Parts of the Indigenous Plant Anabasis aretioïdes Coss. & Moq.

被引：2

作者：

Laaraj, Salah ^{[1
,2
]}

Tikent, Aziz ^{[3
]}

Chebaibi, Mohamed ^{[4
]}

Bouaouda, Khawla ^{[5
]}

Bouhrim, Mohamed ^{[6
,7
]}

Sweilam, Sherouk Hussein ^{[8
]}

Herqash, Rashed N. ^{[9
]}

Shahat, Abdelaaty A. ^{[9
]}

Addi, Mohamed ^{[3
]}

Elfazazi, Kaoutar ^{[1
]}

机构：

[1] Natl Inst Agr Res INRA, Reg Ctr Agr Res Tadla, Agrifood Technol & Qual Lab, Ave Ennasr,Bp 415 Rabat Principal, Rabat 10090, Morocco

[2] Sultan Moulay Slimane Univ USMS, Fac Sci & Technol FST, Environm Ecol & Agroind Engn Lab, LGEEAI, Beni Mellal 23000, Morocco

[3] Univ Mohammed Premier, Fac Sci, Lab Ameliorat Prod Agr Biotechnol & Environm LAPAB, Bp 717, Oujda 60000, Morocco

[4] Minist Hlth & Social Protect, Higher Inst Nursing Profess & Hlth Tech, Fes 30000, Morocco

[5] Univ Hassan II Casablanca, Fac Sci Ben MSik, Lab Biol & Hlth, Casablanca 20650, Morocco

[6] Sultan Moulay Slimane Univ, Fac Sci & Tech, Biol Engn Lab, Beni Mellal 23000, Morocco

[7] Univ Lille, Fac Pharmaceut & Biol Sci, Lab Pharmacol Pharmacokinet & Clin Pharm, Labs TBC, 3,rue Prof Laguesse,BP 83, F-59000 Lille, France

[8] Egyptian Russian Univ, Fac Pharm, Dept Pharmacognosy, Cairo Suez Rd, Cairo 11829, Egypt

[9] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2024年 / 46卷 / 11期

关键词：

southeastern Morocco; indigenous plant; <italic>Anabasis aretio & iuml; des</italic>; antioxidant; antibacterial; anticancer; MEDICINAL-PLANTS; INFLAMMATION; ANTIFUNGAL; MECHANISMS; FLAVONOIDS; RESISTANCE; MOROCCO; SEEDS; RISK; ACID;

D O I：

10.3390/cimb46110735

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anabasis aretio & iuml;des contain numerous bioactive compounds that provide several advantages, including antioxidant, antibacterial, anticancer, neuroprotective, anti-inflammatory, and antidiabetic characteristics. This study aimed to make a hydroethanolic extract from the aerial part of the plant, analyze its biochemical compounds, and test its biological activities. From HPLC-DAD analysis, cinnamic acid, sinapic acid, and vanillin bioactives were found to be the main compounds in the extract. The spectrometric tests revealed that the extract was rich in flavonoids (8.52 +/- 0.32 mg RE/100 g DW), polyphenols (159.32 +/- 0.63 mg GAE/100 g DW), and condensed tannins (8.73 +/- 0.23 mg CE/100 g DW). The extract showed significant antioxidant activity. There were strong correlations between the amount of flavonoid or polyphenol and the antioxidant assays, including ABTS, DPPH, beta-carotene, and TAC. The extract also showed highly effective results against Gram-positive bacteria Staphylococcus aureus and Enterococcus faecalis as well as against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, and showed promising cytotoxicity against breast cancer cell lines MCF-7 and MDA-MB-231. The in silico modeling of the bioactive compounds contained in the extract illustrated their interaction mode with the active sites of particular target proteins, and it showed that rutin had the strongest effect on stopping NADPH oxidase enzyme, with a glide score of -6.889 Kcal/mol. Sinapic acid inhibited E. coli beta-ketoacyl-[acyl carrier protein] synthase (-7.517 kcal/mol), and apigenin showed high binding affinity to S. aureus nucleoside di-phosphate kinase, with -8.656 kcal/mol. Succinic acid has the strongest anticancer effect for caspase-3, with a glide score of -8.102 kcal/mol. These bioactive components may be beneficial as antioxidant and antibacterial applications in medicine, foods, natural cosmetics, and breast cancer prevention in the future. As a result, the use of this indigenous plant must be considered to maximize its value and preservation.

引用

页码：12375 / 12396

页数：22