COMPARISON OF ANTIMICROBIAL, ANTICANCER AND ANTIOXIDANT PROPERTIES OF Fe, Cu, Ag NANOPARTICLES SYNTHESIZED FROM SALVIA OFFICINALIS PLANT

The goal of this study was to comparatively investigate the physicochemical and biological activities of nanoparticles (NPs) formed by the reduction of Salvia officinalis (S. officinalis) using iron (Fe), copper (Cu), silver (Ag) metal salts. Fe@NPs, Cu@NPs, Ag@NPs S. officinalis leaves were synthesized using silver nitrate (AgNO3), copper (II) sulfate (CuSO4), ferric chloride (FeCl2), (FeCl3). Physical-morphological properties of NPs were determined using instrumental analysis techniques FT-IR, UV-visible, SEM, TEM, EDX, XRD and Zeta potential analysis. The antibacterial activity of NPs was evaluated by the disk diffusion method. Total antioxidant, total flavonoid/phenolic capacity, DPPH scavenging activity, metal chelating activities of NPs were also examined. Cytotoxic activity of NPs on A549 lung cancer cell lines was determined in-vitro by WST-1 assay. Fe@NPs 270-290, Cu@NPs 270-320, Ag@NPs 300-400 nm generated strong surface plasmon resonance in the range. According to SEM/TEM results show that Fe@NPs are round-cubic 49.3; Global 30.24 of Cu@NPs; showed that Ag@NPs had circular dimensions of 5,36 nm. Zeta potential values were determined as Fe@NPs -22.2, Cu@NPs 25.5, Ag@NPs -24.6 mV. As a result of antimicrobial analysis, the highest effect was seen in Ag@NPs. Synergetic effects of the extract and metals were evaluated by determining the total antioxidant capacity and total flavonoid/phenolic content of S. officinalis and NPs. DPPH scanvenging activity, BHA (65.89%), S. officinalis, Fe@NPs, Cu@NPs, Ag@NPs; It was evaluated as 79.57%, 72.30%, 75.81%, 81.12%. Against the metal chelating feature of EDTA; S. officinalis was 42.92%, Fe@NPs, Cu@NPs, Ag@NPs were 16.75%, 18.02%, 15.13%. When the results of the anticancer analysis are evaluated; It was determined that Fe@NPs decreased cell viability by 90%, Cu@NPs by similar to 96% and Ag@NPs by 85%. This study reports the successful synthesis of Fe@NPs, Cu@NPs, Ag@NPs with S. officinalis leaf extract. The results show that Fe@NPs, Cu@NPs, Ag@NPs can be used in biomedical applications.