Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms

被引：0
|
作者：
Xu, Hongyu [1 ,2 ]
Jia, Zetian [1 ,2 ]
Liu, Junhui [1 ,2 ]
Liu, Runming [1 ,2 ]
Wei, Wei [1 ,2 ]
Li, Xiang [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Wuhan Univ, Zhongnan Hosp Wuhan Univ, Brain Res Ctr, Wuhan 430071, Peoples R China
[2] Wuhan Univ, Zhongnan Hosp Wuhan Univ, Dept Neurosurg, Wuhan 430071, Peoples R China
[3] Wuhan Univ, Frontier Sci Ctr Immunol & Metab, Wuhan 430072, Peoples R China
[4] Wuhan Univ, Med Res Inst, Wuhan 430072, Peoples R China
[5] Wuhan Univ, Sino Italian Ascula Brain Sci Joint Lab, Wuhan 430071, Peoples R China
来源：
STAR PROTOCOLS | 2025年 / 6卷 / 01期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.xpro.2024.103582
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gut-microbiome-combined metabolomics studies in cerebrovascular disease highlight the microbiota-gut-brain axis in neurological disorders. Here, we present a protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms. We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. We then detail procedures for metabolite extraction, liquid chromatography-tandem mass spectrometry (LC-MS/MS) non-targeted metabolomics sequencing, and ELISA for cerebrospinal fluid and plasma samples. Finally, we perform combined multi-omics analysis. For complete details on the use and execution of this protocol, please refer to Xu et al.1
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页数：20
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